Calcium
Calcium assay (1):
1. Vertical, Light, Root Length Assay
Recommended conditions for phenotypic analysis:
'Low Calcium' - regular MS media with 10 µM EGTA added
'High Calcium' - regular MS media (3 mM Ca), raise to 30 mM Ca with CaCl2
'High Calcium' - regular MS media (3 mM Ca), raise to 30 mM Ca with Ca(NO3)2
'High Calcium' - no MS salts, 30 mM Ca(NO3)2
 |
Media and Controls
Base Media: Calcium preparation,
Control(s): 0.5X MS, 1% Agar, S-,
Correlative Control(s):
References
Comments