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Arabidopsis Gantlet Project
Conditional Phenotype Analysis
Project Description
Reverse genetic studies are often stymied when no atypical phenotypes are observed. In cases where the probable function of the gene is known, it may be possible to design specific and targeted conditions to reveal differences between wild type and mutants. When the gene is a member of a large gene family or when the the function of the gene is uncertain, a broad-spectrum panel of environmental and developmental assays may be needed to uncover conditional phenotypes.
Although it is an obvious approach, gathering the necessary information to run such a screen is time consuming. The general steps required to define each condition are;
1. identify environmental or chemical input,
2. reference published dose response curves if available
3. produce dose response curves for conditions not yet published,
- establish quantifiable trait, i.e. root length,
- graph for wild-type plants, ecotype Wassilevskija (Ws)
- identify threshold doses to test for sensitivity,
- identify saturation doses to test for insensitivity.
The purpose of this WEB site is to collect and publish protocols detailing as broad a range of conditions as is possible for the model plant Arabidopsis thaliana.
Supported by the National Science Foundation.
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